Differentiation of primordial germ cells from premature ovarian insufficiency-derived induced pluripotent stem cells.

ABSTRACT:

Background

Premature ovarian insufficiency (POI) is a common disease in reproductive women. The pathogenesis of POI is not clear, although it is known that it involves the disorder of oocyte differentiation and development. The introduction of reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) offers a unique opportunity to study many aspects of POI from cell differentiation in vitro that could ultimately lead to novel drug development and testing to help treat the disorder.

Methods

The fibroblasts from POI patients, including fragile X syndrome, abnormal karyotype (45, X; 45, X/46, XX; 45, XO and 47, XXX), and the gene mutation (FIGLA and GDF9) were reprogrammed to pluripotency status by retroviral transduction using defined factors. The morpholog

SUBMITTER: Yang S 

PROVIDER: S-EPMC6545034 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature