β-Catenin-Dependent Wnt Signaling: A Pathway in Acute Cutaneous Wounding.

ABSTRACT:

Background

Acute wound healing is a dynamic process that results in the formation of scar tissue. The mechanisms of this process are not well understood; numerous signaling pathways are thought to play a major role. Here, the authors have identified β-catenin-dependent Wnt signaling as an early acute-phase reactant in acute wound healing and scar formation.

Methods

The authors created 6-mm full-thickness excisional cutaneous wounds on adult β-catenin-dependent Wnt signal (BAT-gal) reporter mice. The expression of canonical Wnt after wounding was analyzed using X-gal staining and quantitative real-time polymerase chain reaction. Next, recombinant mouse Wnt3a (rmWnt3a) was injected subcutaneously to the wound edge, daily. The mice were killed at stratified time points, up to 1

SUBMITTER: Carre AL 

PROVIDER: S-EPMC6545117 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature