Development of high oleic peanut lines through marker-assisted introgression of mutant ahFAD2 alleles and its fatty acid profiles under open-field and controlled conditions.

ABSTRACT: Peanut is one of the most important oilseed crops grown worldwide. In this study, the mutant ahFAD2 alleles conferring high oleic (HO) content are introgressed into an elite Indian cultivar GPBD4 which is also resistant to the foliar fungal diseases like rust and late leaf spot (LLS). The allele-specific PCR (AS-PCR) and cleaved amplified polymorphic sequences (CAPS) assays were used for the marker-assisted backcross (MABC) approach and 64 HO introgression lines (ILs) were generated. These ILs were tested for the FA compositions under the glasshouse and field conditions. The oleic acid and linoleic acid contents in the ILs were recorded to be between 68.94-82.33% and 1.74-10.87%, respectively, under glasshouse and 67.04-81.71% and 2.00-15.66%, respectively, under field conditions. T

SUBMITTER: Nawade B 

PROVIDER: S-EPMC6545185 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature