Quantification of cellular protein and redox imbalance using SILAC-iodoTMT methodology.

ABSTRACT: Under normal conditions, the cellular redox status is maintained in a steady state by reduction and oxidation processes. These redox alterations in the cell are mainly sensed by protein thiol residues of cysteines thus regulating protein function. The imbalance in redox homeostasis may therefore regulate protein turnover either directly by redox modulating of transcription factors or indirectly by the degradation of damaged proteins due to oxidation. A new analytical method capable of simultaneously assessing cellular protein expression and cysteine oxidation would provide a valuable tool for the field of cysteine-targeted biology. Here, we show a workflow based on protein quantification using metabolic labeling and determination of cysteine oxidation using reporter ion quantification. We

SUBMITTER: Vajrychova M 

PROVIDER: S-EPMC6545335 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature