Transcriptome and proteome profiling of neural stem cells from the human subventricular zone in Parkinson's disease.

ABSTRACT: It is currently accepted that the human brain has a limited neurogenic capacity and an impaired regenerative potential. We have previously shown the existence of CD271-expressing neural stem cells (NSCs) in the subventricular zone (SVZ) of Parkinson's disease (PD) patients, which proliferate and differentiate towards neurons and glial cells in vitro. To study the molecular profile of these NSCs in detail, we performed RNA sequencing and mass spectrometry on CD271⁺ NSCs isolated from human post-mortem SVZ and on homogenates of the SVZ. CD271⁺ cells were isolated through magnetic cell separation (MACS). We first compared the molecular profile of CD271⁺ NSCs to the SVZ homogenate from control donors and then compared CD271⁺ cells to CD11b⁺

SUBMITTER: Donega V 

PROVIDER: S-EPMC6545684 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature