Characterizing the Collagen Network Structure and Pressure-Induced Strains of the Human Lamina Cribrosa.

ABSTRACT:

Purpose

The purpose of this study was to measure the 2D collagen network structure of the human lamina cribrosa (LC), analyze for the correlations with age, region, and LC size, as well as the correlations with pressure-induced strains.

Methods

The posterior scleral cups of 10 enucleated human eyes with no known ocular disease were subjected to ex vivo inflation testing from 5 to 45 mm Hg. The optic nerve head was imaged by using second harmonic generation imaging (SHG) to identify the LC collagen structure at both pressures. Displacements and strains were calculated by using digital volume correlation of the SHG volumes. Nine structural features were measured by using a custom Matlab image analysis program, including the pore area fraction, node density, and beam connectivi

SUBMITTER: Ling YTT 

PROVIDER: S-EPMC6545820 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature