A chromosome-scale assembly of the major African malaria vector Anopheles funestus.

ABSTRACT:

Background

Anopheles funestus is one of the 3 most consequential and widespread vectors of human malaria in tropical Africa. However, the lack of a high-quality reference genome has hindered the association of phenotypic traits with their genetic basis in this important mosquito.

Findings

Here we present a new high-quality A. funestus reference genome (AfunF3) assembled using 240× coverage of long-read single-molecule sequencing for contigging, combined with 100× coverage of short-read Hi-C data for chromosome scaffolding. The assembled contigs total 446 Mbp of sequence and contain substantial duplication due to alternative alleles present in the sequenced pool of mosquitos from the FUMOZ colony. Using alignment and depth-of-coverage information, these contigs were deduplica

SUBMITTER: Ghurye J 

PROVIDER: S-EPMC6545970 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature