Ripple-related firing of identified deep CA1 pyramidal cells in chronic temporal lobe epilepsy in mice.

ABSTRACT:

Objective

Temporal lobe epilepsy (TLE) is often associated with memory deficits. Reactivation of memory traces in the hippocampus occurs during sharp-wave ripples (SWRs; 140-250 Hz). To better understand the mechanisms underlying high-frequency oscillations and cognitive comorbidities in epilepsy, we evaluated how rigorously identified deep CA1 pyramidal cells (dPCs) discharge during SWRs in control and TLE mice.

Methods

We used the unilateral intraamygdala kainate model of TLE in video-electroencephalography (EEG) verified chronically epileptic adult mice. Local field potential and single-cell recordings were performed using juxtacellular recordings from awake control and TLE mice resting on a spherical treadmill, followed by post hoc identification of the recorded cells.

SUBMITTER: Marchionni I 

PROVIDER: S-EPMC6546014 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature