Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.

ABSTRACT: Prp16 is a DEAH box pre-mRNA splicing factor that triggers a key spliceosome conformational switch to facilitate second step splicing in Saccharomyces cerevisiae. However, Prp16 functions are largely unexplored in Schizosaccharomyces pombe, an attractive model with exon-intron architecture more relevant to several other eukaryotes. Here, we generated mis-sense alleles in SpPrp16 whose consequences on genome-wide splicing uncover its nearly global splicing role with only a small subset of unaffected introns. Prp16 dependent and independent intron categories displayed a striking difference in the strength of intronic 5' splice site (5'SS)-U6 snRNA and branch site (BS)-U2 snRNA interactions. Selective weakening of these interactions could convert a Prp16 dependent intron into an independent o

SUBMITTER: Vijayakumari D 

PROVIDER: S-EPMC6546409 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature