MicroRNA-21-5p dysregulation in exosomes derived from heart failure patients impairs regenerative potential.

ABSTRACT: Exosomes, as functional paracrine units of therapeutic cells, can partially reproduce the reparative properties of their parental cells. The constitution of exosomes, as well as their biological activity, largely depends on the cells that secrete them. We isolated exosomes from explant-derived cardiac stromal cells from patients with heart failure (FEXO) or from normal donor hearts (NEXO) and compared their regenerative activities in vitro and in vivo. Patients in the FEXO group exhibited an impaired ability to promote endothelial tube formation and cardiomyocyte proliferation in vitro. Intramyocardial injection of NEXO resulted in structural and functional improvements in a murine model of acute myocardial infarction. In contrast, FEXO therapy exacerbated cardiac function and left ventric

SUBMITTER: Qiao L 

PROVIDER: S-EPMC6546482 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature