Bio-derived Production of Cinnamyl Alcohol via a Three Step Biocatalytic Cascade and Metabolic Engineering.

ABSTRACT: The construction of biocatalytic cascades for the production of chemical precursors is fast becoming one of the most efficient approaches to multi-step synthesis in modern chemistry. However, despite the use of low solvent systems and renewably-resourced catalysts in reported examples, many cascades are still dependent on petrochemical starting materials, which as of yet cannot be accessed in a sustainable fashion. Herein we report the production of the versatile chemical building block cinnamyl alcohol from the primary metabolite and fermentation product L-phenylalanine. Through the combination of three biocatalyst classes (phenylalanine ammonia lyase, carboxylic acid reductase and alcohol dehydrogenase) the target compound could be reached in high purity, demonstrable at 100 mg scale ach

SUBMITTER: Klumbys E 

PROVIDER: S-EPMC6546598 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature