Bioluminescent-based imaging and quantification of glucose uptake in vivo.

ABSTRACT: Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron-emission tomography imaging remains the gold standard for measuring glucose uptake, and no optical tools exist for non-invasive longitudinal imaging of this important metabolite in in vivo settings. Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo. In addition, we demonstrate that the sensitivity of our method is comparable with that of commonly used ¹⁸F-FDG-positron-emission-tomography tracers and validate t

SUBMITTER: Maric T 

PROVIDER: S-EPMC6546603 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature