Genome-lamina interactions are established de novo in the early mouse embryo.

ABSTRACT: In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome^1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear³. Here we generate high-resolution maps of genomic interactions with the nuclear lamina (a filamentous meshwork that lines the inner nuclear membrane) in mouse pre-implantation embryos. We reveal that nuclear organization is not inherited from the maternal germline but is instead established de novo shortly after fertilization. The two parental genomes establish lamina-associated domains (LADs)⁴ with different features that converge after the 8-cell stage. We find that the mechanism of LAD establishment is unr

SUBMITTER: Borsos M 

PROVIDER: S-EPMC6546605 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature