A Novel Small RNA-Cleaving Deoxyribozyme with a Short Binding Arm.

ABSTRACT: Deoxyribozymes capable of catalyzing sequence-specific RNA cleavage have found broad applications in biotechnology, DNA computing and environmental sensing. Among these, deoxyribozyme 8-17 is the most common small DNA motif capable of catalyzing RNA cleavage. However, the extent to which other DNA molecules with similar catalytic motifs exist remains elusive. Here we report a novel RNA-cleaving deoxyribozyme called 10-12opt that functions with an equally small catalytic motif and an unusually short binding arm. This deoxyribozyme contains a 14-nucleotide catalytic core that preferentially catalyzes RNA cleavage at UN dinucleotide junctions (k_obs = 0.9 h^-1 for UU cleavage). Surprisingly, the left binding arm contains only three nucleotides and forms two canonical base

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6546695 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature