T₂* weighted Deconvolution of NMR Spectra: Application to 2D Homonuclear MAS Solid-State NMR of Membrane Proteins.

ABSTRACT: 2D homonuclear NMR spectroscopy is an essential technique to characterize small and large molecules, such as organic compounds, metabolites, and biomacromolecules at atomic resolution. However, for complex samples 2D homonuclear spectra display poor resolution, making spectral assignment very cumbersome. Here, we propose a new method that exploits the differential T₂* relaxation times of individual resonances and resolves the 2D NMR peaks into pseudo-3D spectra, where time is the 3^rd dimension. T₂* weIghted DEconvolution or TIDE analyzes individual free induction decays (FIDs) and dissects them into sub-FIDs that are transformed into pseudo-3D spectra combining Fourier transformation and covariance NMR. TIDE achieves higher resolution and sensitivity for NM

SUBMITTER: V S M 

PROVIDER: S-EPMC6546711 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature