Topoisomerase IV can functionally replace all type 1A topoisomerases in Bacillus subtilis.

ABSTRACT: DNA topoisomerases play essential roles in chromosome organization and replication. Most bacteria possess multiple topoisomerases which have specialized functions in the control of DNA supercoiling or in DNA catenation/decatenation during recombination and chromosome segregation. DNA topoisomerase I is required for the relaxation of negatively supercoiled DNA behind the transcribing RNA polymerase. Conflicting results have been reported on the essentiality of the topA gene encoding topoisomerase I in the model bacterium Bacillus subtilis. In this work, we have studied the requirement for topoisomerase I in B. subtilis. All stable topA mutants carried different chromosomal amplifications of the genomic region encompassing the parEC operon encoding topoisomerase IV. Using a fluorescent ampli

SUBMITTER: Reuß DR 

PROVIDER: S-EPMC6547408 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature