Global translation inhibition yields condition-dependent de-repression of ribosome biogenesis mRNAs.

ABSTRACT: Ribosome biogenesis (RiBi) is an extremely energy intensive process that is critical for gene expression. It is thus highly regulated, including through the tightly coordinated expression of over 200 RiBi genes by positive and negative transcriptional regulators. We investigated RiBi regulation as cells initiated meiosis in budding yeast and noted early transcriptional activation of RiBi genes, followed by their apparent translational repression 1 hour (h) after stimulation to enter meiosis. Surprisingly, in the representative genes examined, measured translational repression depended on their promoters rather than mRNA regions. Further investigation revealed that the signature of this regulation in our data depended on pre-treating cells with the translation inhibitor, cycloheximide (CHX)

SUBMITTER: Cheng Z 

PROVIDER: S-EPMC6547411 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature