Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.

ABSTRACT: Bacterial genome duplication and transcription require simultaneous access to the same DNA template. Conflicts between the replisome and transcription machinery can lead to interruption of DNA replication and loss of genome stability. Pausing, stalling and backtracking of transcribing RNA polymerases add to this problem and present barriers to replisomes. Accessory helicases promote fork movement through nucleoprotein barriers and exist in viruses, bacteria and eukaryotes. Here, we show that stalled Escherichia coli transcription elongation complexes block reconstituted replisomes. This physiologically relevant block can be alleviated by the accessory helicase Rep or UvrD, resulting in the formation of full-length replication products. Accessory helicase action during replication-transcrip

SUBMITTER: Hawkins M 

PROVIDER: S-EPMC6547429 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature