Replication dynamics of individual loci in single living cells reveal changes in the degree of replication stochasticity through S phase.

ABSTRACT: Eukaryotic genomes are replicated under the control of a highly sophisticated program during the restricted time period corresponding to S phase. The most widely used replication timing assays, which are performed on populations of millions of cells, suggest that most of the genome is synchronously replicated on homologous chromosomes. We investigated the stochastic nature of this temporal program, by comparing the precise replication times of allelic loci within single vertebrate cells progressing through S phase at six loci replicated from very early to very late. We show that replication timing is strictly controlled for the three loci replicated in the first half of S phase. Out of the three loci replicated in the second part of S phase, two present a significantly more stochastic patt

SUBMITTER: Duriez B 

PROVIDER: S-EPMC6547449 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature