Sources of artifact in measurements of 6mA and 4mC abundance in eukaryotic genomic DNA.

ABSTRACT:

Background

Directed DNA methylation on N6-adenine (6mA), N4-cytosine (4mC), and C5-cytosine (5mC) can potentially increase DNA coding capacity and regulate a variety of biological functions. These modifications are relatively abundant in bacteria, occurring in about a percent of all bases of most bacteria. Until recently, 5mC and its oxidized derivatives were thought to be the only directed DNA methylation events in metazoa. New and more sensitive detection techniques (ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-ms/ms) and single molecule real-time sequencing (SMRTseq)) have suggested that 6mA and 4mC modifications could be present in a variety of metazoa.

Results

Here, we find that both of these techniques are prone to inaccuracies, wh

SUBMITTER: O'Brown ZK 

PROVIDER: S-EPMC6547475 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature