Production of trehalose with trehalose synthase expressed and displayed on the surface of Bacillus subtilis spores.

ABSTRACT:

Background

Bacillus subtilis spores have been commonly used for the surface display of various food-related or human antigens or enzymes. For successful display, the target protein needs to be fused with an anchor protein. The preferred anchored proteins are the outer-coat proteins of spores; outer-coat proteins G (CotG) and C (CotC) are commonly used. In this study, mutant trehalose synthase (V407M/K490L/R680E TreS) was displayed on the surface of B. subtilis WB800n spores using CotG and CotC individually or in combination as an anchoring protein.

Results

Western blotting, immunofluorescence, dot blot, and enzymatic-activity assays detected TreS on the spore surface. The TreS activity with CotC and CotG together as the anchor protein was greater than the sum of the enzymati

SUBMITTER: Liu H 

PROVIDER: S-EPMC6547511 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature