Robust hematopoietic specification requires the ubiquitous Sp1 and Sp3 transcription factors.

ABSTRACT:

Background

Both tissue-specific and ubiquitously expressed transcription factors, such as Sp-family members, are required for correct development. However, the molecular details of how ubiquitous factors are involved in programming tissue-specific chromatin and thus participate in developmental processes are still unclear. We previously showed that embryonic stem cells lacking Sp1 DNA-binding activity (Sp1^ΔDBD/ΔDBD cells) are able to differentiate into early blood progenitors despite the inability of Sp1 to bind chromatin without its DNA-binding domain. However, gene expression during differentiation becomes progressively deregulated, and terminal differentiation is severely compromised.

Results

Here, we studied the cooperation of Sp1 with its closest paralogue Sp

SUBMITTER: Gilmour J 

PROVIDER: S-EPMC6547542 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature