A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma.

ABSTRACT:

Background

Circulating microRNAs (miRNAs) are attractive non-invasive biomarkers for a variety of conditions due to their stability and altered pathophysiological expression levels. Reliable detection of global expression profiles is required to maximise miRNA biomarker discovery. Although developments in small RNA-Seq technology have improved detection of plasma-based miRNAs, the low RNA content and sequencing bias introduced during library preparation remain challenging. In this study we compare commercially available RNA extraction methods using MagnaZol (Bioo Scientific) or miRNeasy (QIAGEN) and three library preparation methods - CleanTag (TriLink), NEXTflex (Bioo Scientific) and QIAseq (QIAGEN) - which aim to address one or both of these issues.

Results

Different RNA e

SUBMITTER: Wong RKY 

PROVIDER: S-EPMC6547578 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature