A novel long non-coding RNA from the HOXA6-HOXA5 locus facilitates colon cancer cell growth.

ABSTRACT:

Background

Homeobox A5 (HOXA5), a member of the HOX family, plays an important role in tumor development and morphogenesis, although opposite effects on tumorigenesis have been observed, depending on the tissue type. In this study, we aimed to investigate the role of a novel transcript from the HOXA6-HOXA5 locus in colon cancer tumorigenesis.

Methods

Human colon cancer cell lines were analyzed using next generation sequencing-based targeted mRNA capture. The effects of overexpression and silencing of HOXA5 transcripts were evaluated in vitro and using a xenograft nude mouse model.

Results

We identified three novel transcripts (HOXA5 short, long 1, and long 2) transcribed from the HOXA6-HOXA5 locus in HCT116 colon cancer cells using next generation sequencing-based tar

SUBMITTER: Saijo S 

PROVIDER: S-EPMC6547586 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature