Distinct variations of antibody secreting cells and memory B cells during the course of Kawasaki disease.

ABSTRACT:

Background

Both antibody secreting cells (ASCs) and memory B cells are essential for the maintenance of humoral immunity. To date, limit studies have focused on the two populations in Kawasaki disease (KD). To address the status of humoral immunity during KD, our current concentration is on the variations of ASCs and memory B cells, as well as their subsets in both acute and remission stages of KD.

Methods

ASCs were defined as the population with high expressions of CD27 and CD38 among CD3-CD20- lymphocytes. Based on the expression of surface marker CD138 and intracellular marker IgG, ASCs were further divided into two subsets. Memory B cells were characterized by the expressions of IgD, CD27 and IgM, upon which memory B cells were further categorized into CD27 + IgD- (switc

SUBMITTER: Xu M 

PROVIDER: S-EPMC6547606 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature