StarD5: an ER stress protein regulates plasma membrane and intracellular cholesterol homeostasis.

ABSTRACT: How plasma membrane (PM) cholesterol is controlled is poorly understood. Ablation of the gene encoding the ER stress steroidogenic acute regulatory-related lipid transfer domain (StarD)5 leads to a decrease in PM cholesterol content, a decrease in cholesterol efflux, and an increase in intracellular neutral lipid accumulation in macrophages, the major cell type that expresses StarD5. ER stress increases StarD5 expression in mouse hepatocytes, which results in an increase in accessible PM cholesterol in WT but not in StarD5^-/- hepatocytes. StarD5^-/- mice store higher levels of cholesterol and triglycerides, which leads to altered expression of cholesterol-regulated genes. In vitro, a recombinant GST-StarD5 protein transfers cholesterol between synthetic liposomes. Star

SUBMITTER: Rodriguez-Agudo D 

PROVIDER: S-EPMC6547630 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature