Evaluation of (-)-[18 F]Flubatine-specific binding: Implications for reference region approaches.

ABSTRACT: We aimed to characterize changes in binding of (-)-[18 F]Flubatine to α4 β2 *-nicotinic acetylcholine receptors (α4 β2 *-nAChRs) during a tobacco cigarette smoking challenge. Displacement of (-)-[18 F]Flubatine throughout the brain was quantified as change in (-)-[18 F]Flubatine distribution volume (VT ), with particular emphasis on regions with low VT . Three tobacco smokers were imaged with positron emission tomography (PET) during a 210 min bolus-plus-constant infusion of (-)-[18 F]Flubatine. A tobacco cigarette was smoked in the PET scanner ∼125 min after the start of (-)-[18 F]Flubatine injection. Equilibrium analysis was used to estimate VT at baseline (90-120 min) and after cigarette challenge (180-210 min), at the time of greatest receptor occupancy by nicotine. Smoking reduced VT

SUBMITTER: Bhatt S 

PROVIDER: S-EPMC6547815 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature