Maximum fluorescence and electron transport kinetics determined by light-induced fluorescence transients (LIFT) for photosynthesis phenotyping.

ABSTRACT: Photosynthetic phenotyping requires quick characterization of dynamic traits when measuring large plant numbers in a fluctuating environment. Here, we evaluated the light-induced fluorescence transient (LIFT) method for its capacity to yield rapidly fluorometric parameters from 0.6 m distance. The close approximation of LIFT to conventional chlorophyll fluorescence (ChlF) parameters is shown under controlled conditions in spinach leaves and isolated thylakoids when electron transport was impaired by anoxic conditions or chemical inhibitors. The ChlF rise from minimum fluorescence (F_o) to maximum fluorescence induced by fast repetition rate (F_m-FRR) flashes was dominated by reduction of the primary electron acceptor in photosystem II (Q_A). The subsequent reo

SUBMITTER: Keller B 

PROVIDER: S-EPMC6548062 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature