Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains.

ABSTRACT: The heritability of chromatin states through cell division is a potential contributor to the epigenetic maintenance of cellular memory of prior states. The macroH2A histone variant has properties of a regulator of epigenetic cell memory, including roles controlling gene silencing and cell differentiation. Its mechanisms of regional genomic targeting and maintenance through cell division are unknown. Here, we combined in vivo imaging with biochemical and genomic approaches to show that human macroH2A is incorporated into chromatin in the G1 phase of the cell cycle following DNA replication. The newly incorporated macroH2A retargets the same large heterochromatic domains where macroH2A was already enriched in the previous cell cycle. It remains heterotypic, targeting individual nucleosomes t

SUBMITTER: Sato H 

PROVIDER: S-EPMC6548134 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature