Stable biphasic interfaces for open microfluidic platforms.

ABSTRACT: We present an open microfluidic platform that enables stable flow of an organic solvent over an aqueous solution. The device features apertures connecting a lower aqueous channel to an upper solvent compartment that is open to air, enabling easy removal of the solvent for analysis. We have previously shown that related open biphasic systems enable steroid hormone extraction from human cells in microscale culture and secondary metabolite extraction from microbial culture; here we build on our prior work by determining conditions under which the system can be used with extraction solvents of ranging polarities, a critical feature for applying this extraction platform to diverse classes of metabolites. We developed an analytical model that predicts the limits of stable aqueous-organic interfa

SUBMITTER: Lee UN 

PROVIDER: S-EPMC6548325 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature