ISWI ATPase Smarca5 Regulates Differentiation of Thymocytes Undergoing β-Selection.

ABSTRACT: Development of lymphoid progenitors requires a coordinated regulation of gene expression, DNA replication, and gene rearrangement. Chromatin-remodeling activities directed by SWI/SNF2 superfamily complexes play important roles in these processes. In this study, we used a conditional knockout mouse model to investigate the role of Smarca5, a member of the ISWI subfamily of such complexes, in early lymphocyte development. Smarca5 deficiency results in a developmental block at the DN3 stage of αβ thymocytes and pro-B stage of early B cells at which the rearrangement of Ag receptor loci occurs. It also disturbs the development of committed (CD73⁺) γδ thymocytes. The αβ thymocyte block is accompanied by massive apoptotic depletion of β-selected double-negative DN3 cells and pr

SUBMITTER: Zikmund T 

PROVIDER: S-EPMC6548592 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature