Gasdermin D Protects from Melioidosis through Pyroptosis and Direct Killing of Bacteria.

ABSTRACT: Gasdermin D (GSDMD) cleavage by caspase-1 or caspase-11 inflammasomes triggers pyroptosis, a lytic form of cell death protective against intracellular bacteria. In this study, we examine the role of GSDMD in a mouse model of melioidosis. Gsdmd^-/- mice were more susceptible than wild-type mice to intranasal infection with Burkholderia thailandensis Production of IL-18, but not IL-1β, was decreased in Gsdmd^-/- infected mice. Despite lower IL-18, IFN-γ was produced in similar amounts in wild-type and Gsdmd^-/- mice. In vitro, secretion of both IL-1β and IL-18 by macrophages or dendritic cells infected with B. thailandensis was dependent on GSDMD. Surprisingly, wild-type or GSDMD-deficient neutrophils secreted similar amounts o

SUBMITTER: Wang J 

PROVIDER: S-EPMC6548608 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature