Identification of reference genes for quantitative PCR during C3H10T1/2 chondrogenic differentiation.

ABSTRACT: C3H10T1/2, a mouse mesenchymal stem cell line, is a well-known in vitro model of chondrogenesis that can be easily employed to recapitulate some of the mechanisms intervening in this process. Moreover, these cells can be used to validate the effect of candidate molecules identified by high throughput screening approaches applied to the development of targeted therapy for human disorders in which chondrogenic differentiation may be involved, as in conditions characterized by heterotopic endochondral bone formation. Chondrogenic differentiation of C3H10T1/2 cells can be monitored by applying quantitative polymerase chain reaction (qPCR), one of the most sensitive methods that allows detection of small dynamic changes in gene expression between samples obtained under different experimental co

SUBMITTER: Cappato S 

PROVIDER: S-EPMC6548758 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature