Inhibition of PP2A activity by H₂O₂ during mitosis disrupts nuclear envelope reassembly and alters nuclear shape.

ABSTRACT: Many types of cancer cells exhibit abnormal nuclear shapes induced by various molecular changes. However, whether reactive oxygen species (ROS) induce nuclear deformation has not been fully addressed. Here, we show that hydrogen peroxide (H₂O₂) treatment induced concentration-dependent alterations in nuclear shape that were abolished by pretreatment with the antioxidant N-acetyl-L-cysteine or by catalase overexpression. Interestingly, treatment with H₂O₂ induced nuclear shape alterations significantly more frequently in mitotic cells than in asynchronous cells, suggesting that H₂O₂ mainly affects nuclear envelope disassembly and/or reassembly processes. Because protein phosphatase 2 A (PP2A) activity is reported to be involv

SUBMITTER: Ahn JH 

PROVIDER: S-EPMC6548778 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature