Stepwise Development of an in vitro Continuous Fermentation Model for the Murine Caecal Microbiota.

ABSTRACT: Murine models are valuable tools to study the role of gut microbiota in health or disease. However, murine and human microbiota differ in species composition, so further investigation of the murine gut microbiota is important to gain a better mechanistic understanding. Continuous in vitro fermentation models are powerful tools to investigate microbe-microbe interactions while circumventing animal testing and host confounding factors, but are lacking for murine gut microbiota. We therefore developed a novel continuous fermentation model based on the PolyFermS platform adapted to the murine caecum and inoculated with immobilized caecal microbiota. We followed a stepwise model development approach by adjusting parameters [pH, retention time (RT), growth medium] to reach fermentation me

SUBMITTER: Poeker SA 

PROVIDER: S-EPMC6548829 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature