Quantitative Third Harmonic Generation Microscopy for Assessment of Glioma in Human Brain Tissue.

ABSTRACT: Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to the lack of clear interfaces between the two. The ability of label-free third harmonic generation (THG) microscopy in combination with automated image analysis to quantitatively detect glioma infiltration in fresh, unprocessed tissue in real time is assessed. The THG images reveal increased cellularity in grades II-IV glioma samples from 23 patients, as confirmed by subsequent hematoxylin and eosin histology. An automated image quantification workflow is presented for quantitative assessment of the imaged cellularity as a reflection of the degree of glioma invasion. The cellularity is validated in three ways: 1) Quantitative comparison of THG imaging with fluorescence microscopy of nucleus-s

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC6548968 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature