Aromatic ¹⁹F-¹³C TROSY: a background-free approach to probe biomolecular structure, function, and dynamics.

ABSTRACT: Atomic-level information about the structure and dynamics of biomolecules is critical for an understanding of their function. Nuclear magnetic resonance (NMR) spectroscopy provides unique insights into the dynamic nature of biomolecules and their interactions, capturing transient conformers and their features. However, relaxation-induced line broadening and signal overlap make it challenging to apply NMR spectroscopy to large biological systems. Here we took advantage of the high sensitivity and broad chemical shift range of ¹⁹F nuclei and leveraged the remarkable relaxation properties of the aromatic ¹⁹F-¹³C spin pair to disperse ¹⁹F resonances in a two-dimensional transverse relaxation-optimized spectroscopy spectrum. We demonstrate the applica

SUBMITTER: Boeszoermenyi A 

PROVIDER: S-EPMC6549241 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature