Phosphate removal and recovery using immobilized phosphate binding proteins.

ABSTRACT: Progress towards a more circular phosphorus economy necessitates development of innovative water treatment systems which can reversibly remove inorganic phosphate (P_i) to ultra-low levels (<100 μg L^-1), and subsequently recover the P_i for reuse. In this study, a novel approach using the high-affinity E. coli phosphate binding protein (PBP) as a reusable P_i bio-adsorbent was investigated. PBP was expressed, extracted, purified and immobilized on NHS-activated Sepharose beads. The resultant PBP beads were saturated with P_i and exposed to varying pH (pH 4.7 to 12.5) and temperatures (25-45 °C) to induce P_i release. Increase in temperature from 25 to 45 °C and pH conditions between 4.7 and 8.5 released less than 20% of ad

SUBMITTER: Venkiteshwaran K 

PROVIDER: S-EPMC6549937 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature