Hedgehog signaling regulates osteoblast differentiation in zebrafish larvae through modulation of autophagy.

ABSTRACT: Impaired osteoblast differentiation may result in bone metabolic diseases such as osteoporosis. It was reported recently that hedgehog (Hh) signaling and autophagy are two important regulators of bone differentiation. In order to further dissect their relationship in bone development, we used a zebrafish larvae model to investigate how disruption of one of these signals affects the function of the other and impacts osteoblast differentiation. Our results showed that activation of Hh signaling negatively regulated autophagy. However, suppression of autophagy by knocking down atg5 expression did not alter Hh signaling, but dramatically upregulated the expression of osteoblast-related genes and increased bone mineralization, especially in the den region. On the contrary, inhibition of the Hh

SUBMITTER: Hu Z 

PROVIDER: S-EPMC6550075 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature