Xenotransplantation of Human glioblastoma in Zebrafish larvae: in vivo imaging and proliferation assessment.

ABSTRACT: Glioblastoma (GBM) is the most prevalent type of primary brain tumor. Treatment options include maximal surgical resection and drug-radiotherapy combination. However, patient prognosis remains very poor, prompting the search for new models for drug discovery and testing, especially those that allow assessment of in vivo responses to treatment. Zebrafish xenograft models have an enormous potential to study tumor behavior, proliferation and cellular interactions. Here, an in vivo imaging and proliferation assessment method of human GBM xenograft in zebrafish larvae is introduced. Zebrafish larvae microinjected with fluorescently labeled human GBM cells were screened daily using a stereomicroscope and imaged by light sheet fluorescence microscopy (LSFM); volumetric modeling and composite reco

SUBMITTER: Vargas-Patron LA 

PROVIDER: S-EPMC6550087 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature