De Novo Emergence of Peptides That Confer Antibiotic Resistance.

ABSTRACT: The origin of novel genes and beneficial functions is of fundamental interest in evolutionary biology. New genes can originate from different mechanisms, including horizontal gene transfer, duplication-divergence, and de novo from noncoding DNA sequences. Comparative genomics has generated strong evidence for de novo emergence of genes in various organisms, but experimental demonstration of this process has been limited to localized randomization in preexisting structural scaffolds. This bypasses the basic requirement of de novo gene emergence, i.e., lack of an ancestral gene. We constructed highly diverse plasmid libraries encoding randomly generated open reading frames and expressed them in Escherichia coli to identify short peptides that could confer a benefi

SUBMITTER: Knopp M 

PROVIDER: S-EPMC6550523 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature