Framework for DNA Quantification and Outlier Detection Using Multidimensional Standard Curves.

ABSTRACT: Real-time PCR is a highly sensitive and powerful technology for the quantification of DNA and has become the method of choice in microbiology, bioengineering, and molecular biology. Currently, the analysis of real-time PCR data is hampered by only considering a single feature of the amplification profile to generate a standard curve. The current "gold standard" is the cycle-threshold ( C_t) method which is known to provide poor quantification under inconsistent reaction efficiencies. Multiple single-feature methods have been developed to overcome the limitations of the C_t method; however, there is an unexplored area of combining multiple features in order to benefit from their joint information. Here, we propose a novel framework that combines existing standard curve m

SUBMITTER: Moniri A 

PROVIDER: S-EPMC6551572 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature