Vasopressin Increases Urinary Acidification via V1a Receptors in Collecting Duct Intercalated Cells.

ABSTRACT:

Background

Antagonists of the V1a vasopressin receptor (V1aR) are emerging as a strategy for slowing progression of CKD. Physiologically, V1aR signaling has been linked with acid-base homeostasis, but more detailed information is needed about renal V1aR distribution and function.

Methods

We used a new anti-V1aR antibody and high-resolution microscopy to investigate Va1R distribution in rodent and human kidneys. To investigate whether V1aR activation promotes urinary H⁺ secretion, we used a V1aR agonist or antagonist to evaluate V1aR function in vasopressin-deficient Brattleboro rats, bladder-catheterized mice, isolated collecting ducts, and cultured inner medullary collecting duct (IMCD) cells.

Results

Localization of V1aR in rodent and human kidneys produc

SUBMITTER: Giesecke T 

PROVIDER: S-EPMC6551786 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature