Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation in Saccharomyces cerevisiae.

ABSTRACT:

Background

Expression of d-xylose isomerase having high catalytic activity in Saccharomyces cerevisiae (S. cerevisiae) is a prerequisite for efficient and economical production of bioethanol from cellulosic biomass. Although previous studies demonstrated functional expression of several xylose isomerases (XI) in S. cerevisiae, identification of XIs having higher catalytic activity is needed. Here, we report a new strategy to improve xylose fermentation in the S. cerevisiae strain IR-2 that involves an evolutionary engineering to select top-performing XIs from eight previously reported XIs derived from various species.

Results

Eight XI genes shown to have good expression in S. cerevisiae were introduced into the strain IR-2 having a deletion

SUBMITTER: Seike T 

PROVIDER: S-EPMC6551904 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature