Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging.

ABSTRACT: Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution which is not ideal for cellular force mapping in small platelets. To enable platelet force mapping with submicron resolution, we developed a force-activatable biosensor named integrative tension sensor (ITS) which directly converts molecular tensions to fluorescent signals, therefore enabling cellular force mapping directly by fluorescence imaging. With ITS, we mapped cellular forces in single platelets at 0.4µm resolution. We found that platelet force distrib

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6552678 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature