Rational design of functional materials guided by single particle chemiluminescence imaging.

ABSTRACT: Chemiluminescence (CL) functionalized materials have found tremendous value in developing CL assays for clinical assays and point-of-care tests. To date, the design and optimization of these materials have mainly relied on conventional trial-and-error procedures in which the ensemble performance is evaluated using conditional experiments. Here we have built an optical microscope to acquire the CL emission from single magnetic-polymer hybrid microbeads functionalized with luminol analogues, and to access the CL kinetics of each individual particle. It was incidentally found that a minor subpopulation of microbeads exhibited intense and delayed CL emission while the majority showed transient and weak emission. Structural characterization of the very same individual particles uncovered that t

SUBMITTER: Kong W 

PROVIDER: S-EPMC6553381 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature