Imaging mass spectrometry enables molecular profiling of mouse and human pancreatic tissue.

ABSTRACT:

Aims/hypothesis

The molecular response and function of pancreatic islet cells during metabolic stress is a complex process. The anatomical location and small size of pancreatic islets coupled with current methodological limitations have prevented the achievement of a complete, coherent picture of the role that lipids and proteins play in cellular processes under normal conditions and in diseased states. Herein, we describe the development of untargeted tissue imaging mass spectrometry (IMS) technologies for the study of in situ protein and, more specifically, lipid distributions in murine and human pancreases.

Methods

We developed matrix-assisted laser desorption/ionisation (MALDI) IMS protocols to study metabolite, lipid and protein distributions in mouse (wild-type and ob/

SUBMITTER: Prentice BM 

PROVIDER: S-EPMC6553460 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature