The Genome of C57BL/6J "Eve", the Mother of the Laboratory Mouse Genome Reference Strain.

ABSTRACT: Isogenic laboratory mouse strains enhance reproducibility because individual animals are genetically identical. For the most widely used isogenic strain, C57BL/6, there exists a wealth of genetic, phenotypic, and genomic data, including a high-quality reference genome (GRCm38.p6). Now 20 years after the first release of the mouse reference genome, C57BL/6J mice are at least 26 inbreeding generations removed from GRCm38 and the strain is now maintained with periodic reintroduction of cryorecovered mice derived from a single breeder pair, aptly named Adam and Eve. To provide an update to the mouse reference genome that more accurately represents the genome of today's C57BL/6J mice, we took advantage of long read, short read, and optical mapping technologies to generate a de novo assem

SUBMITTER: Sarsani VK 

PROVIDER: S-EPMC6553538 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature