Simultaneous Ribosome Profiling of Human Host Cells Infected with Toxoplasma gondii.

ABSTRACT: Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. Here, we applied ribosome profiling coupled with mRNA measurements to concurrently study gene expression in the parasite and in host human foreskin fibroblasts. By examining the parasite transcriptome and translatome, we identified potential upstream op

SUBMITTER: Holmes MJ 

PROVIDER: S-EPMC6553554 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature