DNA Rereplication Is Susceptible to Nucleotide-Level Mutagenesis.

ABSTRACT: The sources of genome instability, a hallmark of cancer, remain incompletely understood. One potential source is DNA rereplication, which arises when the mechanisms that prevent the reinitiation of replication origins within a single cell cycle are compromised. Using the budding yeast Saccharomyces cerevisiae, we previously showed that DNA rereplication is extremely potent at inducing gross chromosomal alterations and that this arises in part because of the susceptibility of rereplication forks to break. Here, we examine the ability of DNA rereplication to induce nucleotide-level mutations. During normal replication these mutations are restricted by three overlapping error-avoidance mechanisms: the nucleotide selectivity of replicative polymerases, their proofreading activity, and m

SUBMITTER: Bui DT 

PROVIDER: S-EPMC6553831 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature